- Continuity: When the oracle is asked the future of Sparta, the clip that holds her gown switches from her right shoulder to her left shoulder, then back to the right.
- Continuity: The shadows change positions as Queen Gorgo talks with Theron.
- Continuity: When Queen Gorgo and the Loyalist meet for the first time, they speak against the backdrop of torches. We see their shadows as they talk on the sand. However, when we see them speaking again, the floor beneath them is all stone. It is not possible that their shadows could have reached all the way from where they speaking, down the steps, and out into the sandy courtyard.
- Anacronismi: The Persian cavalry is using stirrups on their horses' saddles. The Battle of Thermopylae was in 480 BC. The earliest representation of a rider using paired stirrups is from a Chinese tomb dated to the 4th century AD. The first use of stirrups recorded in Europe is the 6th century AD, by the Swedish cavalry.
- Anacronismi: In the scenes of Leonidas with his mouth open, you can see that he has fillings in his teeth.
- Incorrectly regarded as goofs: During the battle scenes, the blood never appears on the ground. In one scene, the blood hits the ground and disappears; in many it vanishes in the air. In another, the droplets fall and stick out of the ground like arrows. This is a stylistic choice to reflect the ‘graphic novel’ origins of the film.
- Revealing mistakes: During the scene in which the battle rhino appears, the rhino can be seen passing through one of the Persians.
- Revealing mistakes: After Leonidas kicks the Persian messenger into the pit, he turns and walks towards the camera, when he does this the scar on his right shoulder can be seen coming off slightly.
- Continuity: When Leonidas is carrying the child out of the destroyed village, the child's head is hanging below Leonidas's arm. However, when Leonidas stands in front of the Tree of the Dead, the child's head is level with his arm, and no longer drooping.
- Incorrectly regarded as goofs: In the movie the narrator says the Spartans are descended from Hercules. Hercules is actually the Roman name of the Greek hero, Heracles. This is presumably done because English speakers would most likely recognize the former name then the latter.
- Anacronismi: Several characters have vaccination scars. A very noticeable example is Daxos' left bicep when he and Stelios are looking at the amassed Persian forces and talking about the Spartan's wish for a beautiful death.
- Continuity: After Leonidas' face is cut by the immortals giant, throughout the movie the cut moves slightly (e.g. at first the part under his eye is slightly slanted, then later on in the movie, the cut starts farther down on his face, and it is more straight).
- Personale o equipaggiamento visibile: On a close-up shot of Leonidas, when he is about to kneel to Xerxes, you can see the reflection of the studio lights in his eyes.
- Revealing mistakes: In Xerxes first appearance, all the closeups show prosthetic piercings in his face that are clearly fake attachments with makeup.
- Continuity: The first time the Spartans are attacked, a Spartan's arm is grazed with a spear over the top of the shield wall. When the spear moves forward it cuts the arm, then in the next shot from another angle, the arm has no wound yet and gets cut by the spear retracting.
- Revealing mistakes: The sun sometimes changes position the sky, most noticeably when the Spartans push the Persians off the cliff. In this shot, they are silhouetted against the sun, which is level with them, but seconds later the distant Persians fire arrows which blot out the sun now high above them.
- Continuity: After the Spartans are attacked with the shields for the first time Leonidas stands and breaks the arrows off leaving the heads in his shield. When he turns to attack moments later the arrows are gone completely.
- Continuity: When Leonidas is talking with the Xerxes messenger, you can easily see his plait change position between shots.
- Anacronismi: As Leonidas is considering the Persian Messenger's offer the camera zooms in on a mother standing with her daughter, clearly visible is the curved outline of a modern brassiere cup under the woman's peplos (or chiton).
>>> WARNING: Here Be Spoilers <<<
Goofs below here contain information that may give away important plot points. You may not want to read any further if you've not already seen this title.
- Incorrectly regarded as goofs: SPOILER: Before Astinos dies, you can see the white horse standing in the background through the fog. This is a foreshadowing so the audience knows what is about to happen and realizes the importance of the moment when the Captain finally tells his son what a good job he is doing.
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