The Woody Woodpecker cartoon used in the movie was updated and then used by NASA to explain space travel to the public.
The panoramic view of the lunar scenery was a Chesley Bonestell painting 13 feet long, mounted on wheels and rolled past a stationary camera. To make the stars appear brightly luminous, 534 holes were punched in the painting and illuminated from behind.
This marked the first time that Grace Stafford (cartoon producer Walter Lantz's wife) did the voice of Woody Woodpecker.
In order to make the space suits appear to be in a vacuum they were padded to make them seem inflated. The padding and the studio lights made the suits so hot the actors could wear them for only a few minutes at a time.
The cracked surface of the moon is reminiscent of a dried lake bed. Bonestell knew this was scientifically inaccurate, but used it to give a sense of perspective to the lunar panorama.
A massive campaign was undertaken to create public awareness of the film during its production. This campaign also attracted the attention of independent producer Robert L. Lippert who rushed a low budget space travel film, Rocketship X-M (1950), into production to capitalize on this campaign. Legal action forced Lippert to modify the campaign for his film. Material sent to exhibitors for "Rocketship X-M" carried the disclaimer "This is not 'Destination Moon'." Lippert was able to have "Rocketship X-M" into general release more than three weeks before this film had its preview engagements.